ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (9): 1440-1448.doi: 10.11843/j.issn.0366-6964.2014.09.009

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Effect of Different Storage Conditions on the in vitro Digestibility of Feedstuffs Using a Computer-controlled Simulated Digestion System

MA Sai，CHEN Liang，ZHANG Hong-fu*   

  1. （State Key Laboratory of Animal Nutrition，Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China）
  • Received:2014-03-10 Online:2014-09-23 Published:2014-09-23

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Abstract:

 The effects of storage temperature，packing methods and storage time on the in vitro dry matter digestibility (DMD）and enzymatic hydrolysate gross energy (EHGE）of corn and soybean meal were investigated to provide the basis for the storage of feed samples of computer-controlled simulated digestion system (CCSDS).A 3×2×7 factorial arrangement in a Split plot design was used.The main plot was storage temperature at 4 or -20 ℃ and the sub-plots were packing methods with a zip-lock bag，vacuum package or vacuum & nitrogen filling package and storage time at 0，2，4，6，8，10 or 12 months.The DMD and EHGE of corn and soybean meal were determined by using a computer-controlled simulated digestion system.The results showed as follows：1) The DMD and EHGE of corn and soybean meal stored at -20 ℃ were significantly greater than that stored at 4 ℃ (P<0.05).Compared with the initial values，the variability of the DMD and EHGE of corn and soybean meal stored at -20 ℃ were less than that stored at 4 ℃.2) The DMD of corn and soybean meal stored in the vacuum package or in the vacuum & nitrogen filling package were significantly greater than that stored in the zip-lock bag(P<0.01).The EHGE of corn stored in the vacuum package or in the vacuum & nitrogen filling package were significantly greater than that stored in the zip-lock bag (P<0.01).Compared with the initial values，the variability of the DMD and EHGE of corn and soybean meal stored in the vacuum & nitrogen filling package were less than that stored in the other packing methods.3) The DMD and EHGE of corn and soybean meal decreased as the storage time increased from 0 to 12 months (P<0.01).In conclusion，the DMD and EHGE of corn and soybean meal were affected by the storage temperature，packing methods and storage time.The DMD and EHGE of corn and soybean meal stored in the vacuum & nitrogen filling package at -20 ℃ had low variability and good storage effect.

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